Cbl-b sustained the epithelial phenotype through inhibiting Akt/ERK-miR- 200c-ZEB2 axis in gastric cancer cell. (A) The serum-starved cells were treated with or without IGF-I for indicated times, cell lysates were collected for Western blot analysis. (B) The expression of miR-200c was analyzed by real-time PCR. (C) The expression of miR-200c was performed using microRNA array as described in Materials and methods. Data are means ± SD in three independent experiments. Control group as reference. * ShRNA Cbl-b vs. NS Control, p < 0.05. ShRNA Cbl-b, Cbl-b shRNA transfected; NS Control, Non-silencing controls.