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Figure 6 | Molecular Cancer

Figure 6

From: Estrogen promotes stemness and invasiveness of ER-positive breast cancer cells through Gli1 activation

Figure 6

E2 enhanced the expression of CSC markers in ER-positive MCF-7 cells through Gli1. (A) MCF7 cells were treated with various concentrations (0.1, 1 or 10 nM) of E2 for 4 days. MRNA expression levels of Gli1, Shh, ALDH1, Nanog, SOX2 and Bmi-1 were measured using qRT-PCR, and expression was normalized to that of GAPDH. (B) Western blotting was used to determine the effects of various concentrations of E2 on the expression of Gli1, Shh, ALDH1, Nanog, SOX2 and Bmi-1 proteins in MCF-7 cells. β-Actin was used as a loading control. (C) MCF-7 cells were transfected with vehicle control shRNA (shVEC), shGli1-1 or shGli1-2 and grown in medium with ETOH or E2 for 4 days. RNAs were extracted and assayed using qRT-PCR to determine the expression levels of ALDH1, Nanog, SOX2 and Bmi-1 mRNAs. Bars represent the means ± SEs of three experiments (**, ##: P < 0.01). (D) Effects of E2 on the expression of ALDH1, Nanog, SOX2 and Bmi-1 were estimated using western blotting. β-Actin was used as a loading control.

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Molecular Cancer

ISSN: 1476-4598