Figure 7

E2 enhanced the invasiveness of MCF-7 cells via Gli1. (A) Representative images of wounds at 0 and 48 h in the presence of ETOH or E2. (B) Histograms illustrate relative wound widths at 0 and 48 h. The migration distance of each cell was measured after the photographs were converted to Photoshop files. (C) Matrigel invasion assay. MCF-7 cells were seeded into Matrigel-coated invasion chambers and were treated with ETOH (control) or E2 for 48 h. Representative images of stained invaded cells are shown. Magnification 100×. (D) The number of migrated cells was quantified by counting cells from 10 random fields. Data are representative of three independent experiments. Bars represent the means ± SEs of three experiments (**, ##: P < 0.01).