Figure 4

Silence of FAS enhanced the cytotoxicity of α-mangostin in breast cancer cells. A Breast cancer cells were transfected with siRNA-targeting FAS for 72 h. Equal amounts of total protein from MCF-7 and MDA-MB-231 cells transfected with siRNA-targeting FAS or with the control were subjected to immunoblotting analyses with antibodies against FAS or GAPDH. B Effect of siRNA-induced silencing of FAS expression on cytotoxicity induced by α-mangostin. Cytotoxicity of α-mangostin was enhanced by siRNA-induced reduction of FAS expression levels. Data represented the means ± SD of three independent experiments. *p < 0.05, **p < 0.01 compared with the same concentration of α-mangostin in control-transfected cells.