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Figure 4 | Molecular Cancer

Figure 4

From: A hormone-dependent feedback-loop controls androgen receptor levels by limiting MID1, a novel translation enhancer and promoter of oncogenic signaling

Figure 4

AR mRNA and protein turnover in response to MID1 or α4. (A) Real-Time PCR analysis of AR mRNA stability in LNCaP cells treated with non-silencing (ns), α4 or MID1 specific siRNA oligonucleotides. Levels of AR relative to GAPDH mRNA were plotted against time points after ActinomycinD addition. (B) Real-Time PCR analysis of AR mRNA stability in fibroblast cells harboring mutant MID1 (OS17/98) and control fibroblasts (C18/98). Levels of AR relative to GAPDH mRNA was plotted against time points indicating ActinomycinD addition (n = 3). (C) Western blot analysis detecting AR and GAPDH proteins after knockdown of α4 in LNCaP cells. Samples were taken at indicated time points after cycloheximide addition (n = 3). Densitometric analysis of Western blots is shown (bottom panels). (D) Western blot analysis detecting AR and GAPDH proteins after over-expression of FLAG-MID1 (MID1) in PC-3 cells with ectopically expressed AR. Samples were taken at indicated time points after cycloheximide addition. Densitometric analysis of Western blots is shown (bottom panels).

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