Figure 2
V158411 induces caspase activation and cell death in human leukemia and lymphoma cell lines. (A) Treatment with 5-times the GI50 of V158411 for 24 or 48 hours activated caspase-3/7 dependent apoptosis. Values are the average of 3 determinations ± SD. (B) zVAD-FMK blocks V158411 induced caspase 3/7 activation. Cells were treated with 5- or 10-times the GI50 of V158411 in the absence or presence of 25 μM zVAD-FMK. Values are the average of 3 determinations ± SD. (C) Cell death in leukemia and lymphoma cell lines induced by V158411 can occur independently of caspase-3/7 activity. Cells were treated with 5-times the GI50 of V158411 in the absence or presence of 25 μM zVAD-FMK for 24 hours. Values are the average of 3 determinations; error bars have been removed for clarity. (D) Continual treatment is more effective in inducing cell death in U937 lymphoma cells than pulsed treatment. U937 cells were treated with 5- or 20-times the GI50 of V158411 either continually for 96 hours (left) or a 24 hour pulse (right). Values are the average of 3 determinations ± SD.