AKR1C2 and 3 expressions in senescence and post-senescence emergence. (A) The mRNA levels of AKR1C2 and 3 were measured by RT-qPCR in extracts from young (Y), senescent (S) and emergent (E) NHEKs obtained from three different donors. AKRC2 and 3 mRNA levels were normalized to 18S levels (Student t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). (B) AKR1C2 and 3 protein levels were evaluated by Western Blot in extracts from young (Y), senescent (S) and emergent (E) NHEKs. The gel was equicharged with extracts from an equal number of cells. The equicharged was verified a posteriori by detecting the levels of GAPDH.