Figure 4
Certain clinicopathological features were associated with the demethylation status of FOXP3 -TSDR in tissue samples. Associations between DMRs and Gender and Distant metastasis (M) were shown. A higher TSDR-DMR was detected in normal tissues (DMRN) both in female patients (p = 0.001, Figure 4B) and those with distant metastases (M1) (p = 0.017, Figure 4D). Either for Gender or for Distant metastasis, no correlation was found to be with the TSDR-DMR in tumor nests (DMRT) (Figure 4A, 4C). Mann-Whitney U tests were applied, and a two-tailed p value ≤0.05 was considered statistically significant.