Protocol for bortezomib treatment and follow-up. The chart illustrates male mice who at 35 days of age were treated with one 11-day-cycle of bortezomib (1 mg/kg, i.p; injection days indicated: d1, d4, d8 and d11) and then followed for 2 days, 45 days, or 6 months. For mating study, male mice at the same age were treated with bortezomib or vehicle as mentioned above. Six months after these mice and their controls were mated with 8–9 week-old healthy females and killed one day later. The resulting pups were sacrificed on postnatal day 50.