HOTAIR expression in gallbladder cancer and its clinical significance. (A) Difference in expression levels of HOTAIR expression levels between gallbladder cancer tissues and matched non-tumor gallbladder tissues. The expression of HOTAIR was normalized to GADPH. The statistical differences between samples were analyzed with paired samples t-test (n = 65, p < 0.0001). (B) Relationship between HOATIR expression and primary tumor growth (p < 0.0001). (C) Relationship between HOATIR expression and lymph node metastasis (p < 0.0001). (D) Expression level of HOTAIR in four gallbladder cancer cell lines. The statistical differences between groups were analyzed using independent samples t-test. *p < 0.05; **p < 0.01.