HOTAIR’s oncogenic activity is in part through negative regulation of miRNA-130a. Representative images (A) and the number of migratory cells (B) per high-power field transfected with si-NC, si-HOTAIR, miR-130a inhibitor or si-HOTAIR + miR-130a inhibitor. The migratory ability of GBC-SD cells can be blocked by HOTAIR downregulation. The si-HOTAIR-blocked migratory ability of GBC-SD cells was rescued by miR-130a inhibitor. Quantitative graphical representation of Apoptosis, G1, G2, S cell population (C) and a bar-graphical representation S-Phase Fraction cells in each group (D) transfected with si-NC, si-HOTAIR, miR-130a inhibitor or si-HOTAIR + miR-130a inhibitor. Si-HOTAIR induced a reduction of S-Phase Fraction cells in GBC-SD cells, which can be rescued by miR-130a inhibitor. Error bars represent S.E.M., n = 3. *p < 0.05; **p < 0.01.