Figure 9

Expression of HOTAIR, c-Myc and miRNA-130a mRNA levels in human gallbladder cancer samples. (A) c-Myc is upregulated in gallbladder cancer tissues compared with paired adjacent normal gallbladder tissues. c-Myc mRNA expression was analyzed by real-time PCR and normalized to GADPH. The statistical differences between samples were analyzed with paired samples t-test (n = 65, p < 0.0001). (B) The correlation between HOTAIR and c-Myc expression levels in gallbladder cancer tissues and matched adjacent normal gallbladder tissues (n = 65). Quantitative RT-PCR was performed in triplicate for each sample and assays were repeated once. The relative levels were normalized to GADPH. Each point in the scatter graph represents an individual sample, in which relative c-Myc levels indicate on x-axis and HOTAIR levels on y-axis. The x-axis shows normalized Ct values for c-Myc determined by quantitative RT-PCR. The y-axis shows normalized Ct values for HOTAIR determined by quantitative RT-PCR. “Mean of normalized Ct values” is the subtraction of “mean of triple Ct values for c-Myc (x-axis) or HOTAIR (y-axis)” by “mean of triple Ct values for GADPH”. The correlation coefficient, R = 0.7063, p <0.0001, indicates there is a strongly positive relationship between c-Myc and HOTAIR. (C) miRNA-130a is downregulated in gallbladder cancer tissues compared with paired adjacent normal gallbladder tissues. miRNA-130a mRNA expression was analyzed by real-time PCR and normalized to GADPH. (D) The correlation between HOTAIR and miR-130a expression levels in gallbladder cancer tissues and matched adjacent normal gallbladder tissues (n = 65). The correlation coefficient, R = -0.6398, p <0.0001, indicates there is a strongly negative relationship between miRNA-130a and HOTAIR.