Downregulation of deoxycytidine kinase in cytarabine-resistant mantle cell lymphoma cells confers cross-resistance to nucleoside analogs gemcitabine, fludarabine and cladribine, but not to other classes of anti-lymphoma agents

Table 1 List of proteins differentially expressed in MINO R cells identified by 2-DE

Spot no.	Accession	Protein name	Fold change	Mascot score	Sequence cov. (%)	Mr
Proteins downregulated in MINO R cells
1	P27707	Deoxycytidine kinase	−4.6	44^*	16	30841
2	Q99829	Copine-1	−4.3	102	17	59649
3	P13796	Plastin-2	−2	453	65	70814
Proteins upregulated in MINO R cells
4	P07741	Adenine phosphoribosyltransferase	5	70	40	19766
5	P68363	Tubulin alpha-1B chain	5	169	32	50804
6	P04792	Heat shock protein beta-1	2/3	73	32	22826
7	P31937	3-Hydroxyisobutyrate Dehydrogenase, Mitochondrial	2/1	43*	8	35712

*Identity of proteins with low Mascot Score was verified by MS/MS (see Table 2).
Included are the proteins with difference in expression at least 2-fold and statistical significance of the change p < 0.05. Swiss-Prot no. is the code under which the identified protein is deposited in the Swiss-Prot database. Mascot score helps to estimate the correctness of the individual hit. It is expressed as −10 × log(P) where P is the probability that the observed match is a random event. Sequence coverage is the number of amino acids spanned by the assigned peptides divided by the sequence length.

ISSN: 1476-4598