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Figure 1 | Molecular Cancer

Figure 1

From: Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer

Figure 1

Breast cancer cells expressing LSR are sensitive to iota toxin. (A) Representative breast cancer cell lines were grown to 80% confluence, lysates isolated, and then analyzed via western blot using LSR and CD44 specific antibodies; α-tubulin was used as a loading control. Representative western blot and (B) corresponding intensity measured via ImageJ. Data represent mean relative intensity +/− SE. Cells were treated with Ia or Ia + Ib for 8 h in the absence (C) and presence (D) of 0.8 mM oleic acid in normal growth media for 20 min at 37°C prior to treatment. Representative images demonstrate cell rounding and detachment from tissue culture dish, indicative of cell death. (E) Cells were treated with Ia or Ia + Ib in complete growth medium at 37°C and cell death was quantified at the indicated times via fluorescent cytotoxicity assay. *P < 0.01. A minimum of three independent experiments was performed for each analysis. RFU; relative fluorescent units.

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Molecular Cancer

ISSN: 1476-4598