Figure 3

miR-200c regulated the epithelial-mesenchymal transition (EMT). (A) SPC-A-1 cells were transfected with miR-control inhibitor or miR-200c inhibitor lentiviral vector, and SPC-A-1sci cells were transfected with the miR-control or miR-200c lentiviral vector. Light microscope pictures were taken after transfection (100x magnification). (B) The protein levels of E-cadherin (epithelial marker) and N-cadherin (mesenchymal marker) were determined by western blot analyses after transfection with miR-control inhibitor or miR-200c inhibitor lentiviral vector, and SPC-A-1sci cells were transfected with the miR-control or miR-200c lentiviral vector. β-actin served as an internal control. (C) Immunofluorescence staining of E-cadherin, N-cadherin, and Phalloidin, after transfection with miR-control inhibitor or miR-200c inhibitor lentiviral vector, and SPC-A-1sci cells were transfected with the miR-control or miR-200c lentiviral vector. The red, green signal represents the staining of the indicated proteins, and the blue signal represents the nuclear DNA staining by DAPI. Magnification, 600×. The results are representative of at least three independent experiments.