IEC-specific constitutive expression of claudin-1 in APCMinmice increases colon tumorigenesis and decreases survival. (A) APCMin mice were crossed with Cld-1Tg mice and colon tumors were quantitated from littermate of APCMin and APC-Cldn1 mice (n = 18 APC mice, n = 17 APC-Cldn1 mice; p = 0.0003). (B) Representative images of the colon from APCMin and APC-Cldn1 mice showing increased sporadic colon tumors. (C) Tumor area was measured in APCMin and APC-Cldn1 mice (p = 0.0178). (D) Representative H&E staining. (E) Invading carcinoma in APC- Cldn1 mouse. (F) Kaplan Meir survival curve demonstrating survival of APCMin (n = 43) and APC-Cldn1 (n = 40) mice (p = 0.0027). Values are mean ± S.E.M.