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Figure 5 | Molecular Cancer

Figure 5

From: HOXA9 promotes homotypic and heterotypic cell interactions that facilitate ovarian cancer dissemination via its induction of P-cadherin

Figure 5

Reconstitution of P-cadherin in HOXA9-knockdown EOC cells restores the stimulatory effects of HOXA9 on cell survival, implantation and migration in vitro. (A) Western blot analysis of P-cadherin levels in HOXA9+ control SKOV3ip cells (non-targeting), HOXA9-knockdown SKOV3ip cells (shA9-B) and HOXA9-knockdown SKOV3ip cells that stably expressed P-cadherin (shA9-B + PCAD). (B) Morphology of SKOV3ip cells following incubation as suspension cultures in polyHEMA-coated plates for 3 days. Bar, 100 μm. (C) Evaluation of cell death in suspension cultures by flow cytometric analysis of 7AAD staining. (D) Evaluation of cell death by ELISA in suspension cultures of SKOV3ip cells, attachment of SKOV3ip cells to confluent mesothelial cell monolayers, and migration of SKOV3ip cells. Shown in D are mean + sd values of three independent assays.

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Molecular Cancer

ISSN: 1476-4598