Figure 5

MONC leads the expansion of immature erythroid precursors. A) qRT-PCR of MONC-transduced CD34⁺-HSPCs. B) Numbers of megakaryocytic (CD41⁺) and non-megakaryocytic (CD41⁻) CFUs from Megacult® assays of transduced HSPCs after 14 days (n = 2). C) Number of colonies in the methocellulose-based CFU-assay of transduced HSPCs after 12 days (n = 2). D) Representative flow cytometry dot plots of transduced HSPCs stained for CD71 and CD117 on day 4 of in vitro culture (n = 2). E) Representative flow cytometry histogram of CD13-stained transduced HSPCs on day 4 of in vitro culture (n = 2). Mean fluorescence intensities (MFI) are indicated. F-G) Representative flow cytometry dot plots of transduced HSPCs stained for F) CD36 and CD235a and G) stained for CD41 and CD42b after 7 days of mixed erythroid-megakaryocytic differentiation culture (n = 2); population frequencies with errors are displayed for respective framed gates. (A-G) Data are presented as mean ± s.d. *P < 0.05; **P < 0.01.