Figure 3
From: Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells
Human Dicer1e. (A) The Dicer1e cDNA sequence and its deduced amino acid sequence [GenBank: KJ175111]. The largest open reading frame encodes an 820 amino acid protein with a predicted molecular mass of ~93-kDa. The underlined nucleotides are the upstream in-frame stop codons, the translational methionine start site, the siDicer1e sense sequence, and the translational stop codon, respectively. The nucleotides in bold type represent the locations of the Dicer1/1e-specific sense primer (Dic1/1e3S1) and the Dicer1e-specific antisense/sense primers (Dic1e5AS and Dic1e3S2), respectively. The underlined amino acid residues are the two ribonuclease III (RNase III) domains. The amino acid residues highlighted by a box are the predicted nuclear localization signal (NLS). The amino acid residues in bold type represent a unique sequence of residues not present in the Dicer1 protein (Unique) and the double-stranded RNA binding domain (dsRBD), respectively. (B) Schematic representation of human Dicer1 protein in comparison to Dicer1e. Individual protein domain structures are indicated by different shadings. Helicase, ATPase/helicase; DUF283, domain of unknown function; PAZ, Piwi/Argonaute/Zwille.