Figure 5

Knockdown of Dicer1e impairs cell proliferation and clonogenicity of oral cancer cells. (A) Western blot analysis of Dicer1 and Dicer1e protein levels in human OSCC cell lines (CAL 27, SCC-4, and SCC-25) 48 hours post-transfection with either control non-targeting siRNA (siNT) or siRNA targeting Dicer1e (siDicer1e). GAPDH was used as a loading control. (B) Cell proliferation assay was performed in OSCC cell lines CAL 27, SCC-4, and SCC-25, after treatment with siDicer1e compared to control siNT-treated cells. Data are mean ± SEM of three separate experiments performed in triplicate, where *P < 0.05 compared to siNT treated cells (Student’s t Test). (C) Assessment of clonogenic potentials of the siDicer1e-treated oral cancer cell lines CAL 27, SCC-4, and SCC-25, compared to control siNT-treated cells. The number of colonies were counted and the data are presented as mean ± SEM of three separate experiments performed in duplicate, where ****P < 0.0001, *P < 0.05 compared to siNT treated cells (Student’s t Test).