Figure 6
From: Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells
Silencing of Dicer1e induces apoptosis and/or G2/M cell cycle arrest in oral cancer cells. (A) Western blot analysis of PARP and caspase-3 cleavage levels in human OSCC cell lines (CAL 27, SCC-4, and SCC-25) 48 hours post-transfection with either control non-targeting siRNA (siNT) or siRNA targeting Dicer1e (siDicer1e). Dicer1e and GAPDH levels were monitored to ensure knockdown and equal loading of samples, respectively. (B) Cell cycle analyses of SCC-4 and SCC-25 cells after treatment with siDicer1e compared to control siNT-treated cells. Data are mean ± SEM of three independent experiments, where *P < 0.05 compared to siNT treated cells (Student’s t test). (C) Western blot analysis of p53 protein levels in CAL 27, SCC-4, and SCC-25 cell lines 48 hours post-transfection with either control siNT or siDicer1e. GAPDH was used as a loading control.