Figure 1

Differences between A549/CDDP cells and parental A549 cells are shown. (A) A549/CDDP cells exhibiting fibroblastic morphology and the A549 cells showing epithelial-like appearance (original magnification, ×100). (B) MTT assay shows that A549/CDDP cells are much more resistant to cisplatin than A549 cells. (C) Western blotting illustrates reduced expression of E-cadherin and increased expression of vimentin in A549/CDDP cells. β-actin was used as an internal control. (D) Invasion assay reveals significant enhancement of invasion ability of A549/CDDP cells in vitro. (E) qRT-PCR indicates a significant up-regulation of miR-27a in A549/CDDP cells compared with A549 cells. miRNA abundance was normalized to U6 RNA. Data are means of three separated experiments ± SD; *P < 0.01.