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Figure 3 | Molecular Cancer

Figure 3

From: Transducin β-like 1 X-linked receptor 1 suppresses cisplatin sensitivity in Nasopharyngeal Carcinoma via activation of NF-κB pathway

Figure 3

NPC Cells overexpressing TBL1XR1 proteins are less sensitive to cisplatin-induced apoptosis. (A) Overexpression of TBL1XR1 in NPC cell lines. Western blotting analysis of TBL1XR in SUNE1-vector, SNUE-TBL1XR1, CNE2-vector and CNE2-TBL1XR1 cells. GAPDH was used as loading control. (B) SUNE1-vector, SNUE-TBL1XR1, CNE2-vector and CNE2-TBL1XR1 cells treated by cisplatin (5 μg/ml, 10 μg/ml, 15 μg/ml, 20 μg/ml) for 48 hours. MTT analysis of the proportion of cells still alive after treatment. (C) Quantification of TUNEL positive cells. SUNE1-vector, SNUE-TBL1XR1, CNE2-vector and CNE2-TBL1XR1 cells were treated by cisplatin (20 μg/ml) for 24 h, followed by TUNEL staining and the number of TUNEL-positive cells was counted from 10 random fields of at least 500 cells. Results are expressed as percentages of total cells. (D) Flow Cytometry analysis of Annexin V+/PI¯ cells after the indicated cell lines treated with cisplatin (20 μg/ml) for 24 h. Results are expressed as percentages of total cells. * P ≤ 0.05.

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Molecular Cancer

ISSN: 1476-4598