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Figure 4 | Molecular Cancer

Figure 4

From: Transducin β-like 1 X-linked receptor 1 suppresses cisplatin sensitivity in Nasopharyngeal Carcinoma via activation of NF-κB pathway

Figure 4

Downregulation of endogenous TBL1XR1 expression decreases the resistance of NPC cells to apoptosis. (A) TBL1XR1 knockdown was achieved by introducing specific shRNA in NPC cells. Western blotting analysis of TBL1XR in SUNE1-vector, SNUE-TBL1XR1-Ri, CNE2-vector and CNE2-TBL1XR1-Ri cells. GAPDH was used as loading control. (B) SUNE1-vector, SNUE-TBL1XR1-Ri, CNE2-vector and CNE2-TBL1XR1-Ri cells treated by cisplatin (5 μg/ml, 10 μg/ml, 15 μg/ml, 20 μg/ml) for 48 hours. MTT analysis of the proportion of cells still alive after treatment. (C) Quantification of TUNEL positive cells. SUNE1-vector, SNUE-TBL1XR1-Ri, CNE2-vector and CNE2-TBL1XR1-Ri cells were treated by cisplatin (20 μg/ml) for 24 h, followed by TUNEL staining and the number of TUNEL-positive cells was counted from 10 random fields of at least 500 cells. Results are expressed as percentages of total cells. (D) Flow Cytometry analysis of Annexin V+/PI¯ cells after the indicated cell lines treated with cisplatin (20 μg/ml) for 24 h. Results are expressed as percentages of total cells. * P ≤ 0.05.

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Molecular Cancer

ISSN: 1476-4598