Figure 6

GzB over-expression increases the sensitivity of Karpas 299 cells to staurosporine-induced apoptosis. A. Representative flow cytometry plot showing GzB expression in Karpas 299 cells transfected with either a GzB cDNA (red line) or vector alone blue line). The shaded histogram indicates cells stained with an irrelevant isotype control antibody. The numbers above each peak represent the mean fluorescence intensity of GzB staining in vector and GzB cDNA transfected cells. B. Cells in A. were treated for 6 h with staurosporine at the indicated concentrations and apoptosis was measured by TUNEL. The results represent the average and standard deviation of 5 independent experiments. p values comparing cells transfected with GzB cDNA to cells transfected with vector alone were obtained by performing paired, one-tailed t-tests. *p < 0.05, § p < 0.01.