Figure 1

Schematic of different chimeric antigen receptors (CARs) used to re-direct the T cell immune response. (A) Schematic structure of second-generation classic CAR. Second-generation CARs contain one costimulatory endodomains (illustrated with CD28 or 4-1BB or OX-40 or CD27), cloned in frame with the scFv and the CD3z endodomain. (B) Schematic structure of physiological CAR which contains full length CD27 or NKG2D receptor fused to CD3z endodomain. (C) Schematic structure of universal CAR, which utilize biotin or anti-FITC scFv as binding domain fused costimulatory and CD3z endodomains.