mRNA and protein expression level of THBS2 in 14 gastric cancer tissues and the corresponding normal tissue. (A) Relative mRNA expression of THBS2 was detected by quantitative real-time PCR. Expression of normal tissues were normalized to 1.0 as indicated. (B) The densitometry analysis results of the THBS2 bands were normalized to GAPDH using Image J. Expression of normal tissues were normalized to 1.0 as indicated. (C) Relative protein expression of THBS2 was estimated by Western blot assay.