Overexpression of THBS2 inhibited the proliferation of gastric cancer cells, suppressed the angiogenesis of gastric cancer, and promoted apoptosis in SGC-7901 cell line in vitro . (A) Western blot analysis of Flag in 293 T cells with THBS2 overexpression. (B) Colony number analysis for MKN-45 and SGC-7901 cells with THBS2 overexpression and negative control. Values are means ± SD from three independent experiments. (C) Images of colony formation assay. (D) AngiogenicIndexCh1 analysis of endothelial cell tube formation assay. Values are means ± SD. (E) Images of endothelial cell tube formation assay. (F) Analysis of apoptosis in MKN-45 cell line. Values are means ± SD.