Figure 5
From: Targeting DNA-PKcs and telomerase in brain tumour cells
Inhibition of DNA-PKcs and telomerase increases cell death. (A) Greatest reduction in cell viability was observed following dual inhibition of telomerase and DNA-PKcs with 1.0 μM MST-312 and 10 μM NU7026 respectively for 48 hours in MO59K cells. (B) Synergistic reduction in cell number observed in MST-312 and NU7026 treated cells for one week determined by trypan blue exclusion assay. *P-value <0.05 when compared to untreated controls. (C) Partial metaphase spreads from MO59K cells displaying chromosomes stained for telomeres (red) and centromeres (green) with chromosomal fusion doublets (1), absence of telomere signals (2), different telomere intensity between sister chromatids (3). (D) Number aberrant telomeres per cell in DNA-PKcs inhibited MO59K cells after 1.0 μM MST-312 treatment for 1 week.