Figure 2

ADAM17 regulates cellular viability, migration, adhesion and proliferation. A: SCC-9 cells stably expressing ADAM17-HA or FLAG-GFP were seeded in 96-well plates. After 7 days cell viability was measured by MTT assay. Three independent experiments were performed (n = 3, Student’s t-test, p = 0.0004). B: SCC-9 cells stably expressing ADAM17-HA or FLAG-GFP were seeded in serum free media in the upper chamber of 96-well transwell plates. EGF at concentration of 100 ng/ml was added in serum free media in the lower chamber (n = 3, Student’s t-test, p = 0.0316). C: SCC-9 cells stably expressing ADAM17-HA or FLAG-GFP were seeded in Matrigel coated 96-well plates. After 1 h, cells were stained and adhesion measured (n = 3, Student’s t-test, p = 0.0001). D: ADAM-17 knockdown decreased adhesion of A431 cells. A431/untreated (mock), A431/control (scrambled) and A431/shRNA ADAM-17 cells were seeded in Matrigel coated 96-well plates. After 1 h, cells were stained and the cell adhesion was measured (n = 3, distinct letters represent significant differences at p < 0.0003, ANOVA followed by Tukey test). E: ADAM-17 knockdown decreased proliferation of A431 cells. Proliferation assay was performed in A431/control (scrambled) and A431/shRNA ADAM-17 cells by measuring BrdU incorporation into DNA in the presence of 2% or 10% FBS (n = 1, quintuplicate, distinct letters represent significant differences at p < 0.05, ANOVA followed by Tukey test).