Figure 3
From: ADAM17 mediates OSCC development in an orthotopic murine model
SCC-9 cells overexpressing ADAM17-HA increased tumor size and proliferation. (A) SCC-9 cells overexpressing ADAM17-HA (AD17-HA) induce increased size of tumors in Nude mice. Cells grown in tissue culture plates were trypsinized, resuspended in PBS and injected in the tongue of Nude mice. After 20 days, mice were sacrificed, tumors excised and measured (n = 3, Student’s t-test, p = 0.0467). (B) SCC-9 cells overexpressing ADAM17-HA (AD17-HA) induce higher proliferative activity by immunohistochemical expression of Ki-67. Positive cells were calculated by counting labeled nuclei (positive-cells) of six high power fields (magnification of 400×) from each case with the aid of the Image J software and expressing the data as percentage (n = 6, Student’s t-test, p < 0.0001).