Figure 4

HE4 and ANXA2 specific binding promotes ovarian cancer cell invasion and migration. A, The immunoblot shows the expression of HE4 and ANXA2 in ovarian cancer cells after ANXA2 transfection. Lanes 1 and 4: ANXA2 low expression groups in ES-2 and CaoV-3 cells; lanes 2 and 3: mock groups. Quantitative data in panel B are expressed as ANXA2 and HE4 relative to GAPDH. Real time PCR results (C) show the expression of HE4 and ANXA2 in each group. The migratory (D, E, original magnification × 40) and invasive (F, original magnification × 200) capacities of ovarian cancer cells before and after transfection and extrinsic HE4 active protein supplementation. Lane 1, mock groups; lane 2, ANXA2 low expression groups; lane 3, ANXA2 low expression cells treated with HE4 active protein; lane 4, ANXA2 high expression groups; lane 5, ANXA2 high expression cells treated with HE4 active protein; lane 6, antibody-mediated blocking of annexin II; lane 7, antibody-mediated blocking of ANXA2 expression with HE4 active protein treatment.