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Figure 4 | Molecular Cancer

Figure 4

From: Sensitivity of leukemic T-cell lines to arsenic trioxide cytotoxicity is dependent on the induction of phosphatase B220/CD45R expression at the cell surface

Figure 4

Subcellular localization of constitutively expressed and As 2 O 3 -induced B220/CD45R molecules. Untreated and As2O3-treated (2 μM for 24 h) EL-4 cells (panel A), and untreated L1210 cells (panel B) were stained with PE-conjugated anti-B220/CD45R mAb and DAPI nuclear dye. Samples were then analyzed on the ImageStream system. At least 10,000 images were collected per sample at 40× and 60× magnification. B220-PE positive cells were gated (gate R2) according to the principle for flow cytometry as shown on histogram. Background staining was determined using PE-conjugated rat IgG2a isotype control. Representative cell images of brightfield illumination, B220-PE (red) and DAPI (blue) fluorescence and composite images of B220/DAPI are shown on panel A and B.

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Molecular Cancer

ISSN: 1476-4598