Differentiation potential of murine adipose derived mesenchymal stem cells (ADSCs). A) Isolated ADSCs at passage 6. B) Differentiation of murine ADSCs into adipocytes. Oil red O staining visualizes lipid vacuoles. C and D) Cartilage differentiation of ADSCs. Alcian blue (C) and Toluidin blue staining (D) indicate successful differentiation of ADSCs into chondrocytes. Inlay shows negative control for Alician blue staining. E and F) Osteogenic differentiation was confirmed by Alizarin red S (E) and Von Kossa staining (F). Red coloring in Alizarin red S and black nodules in Von Kossa staining respectively, indicative of calcium and extra matrix deposits produced by ADSCs. ADSC = adipose derived mesenchymal stem cell.