Representative fluorescence-activated cell sorting (FACS) analysis of surface markers expressed by murine ADSCs cultured at Passage 6. One experimental representative of three is shown. Cells were incubated with different monoclonal antibodies conjugated with either fluorescein isothiocyanate (FITC) or phycoerythrin (PE). White histograms correspond to cells stained with antibodies against mouse surface epitopes. Staining with the appropriate IgG isotype control antibody is shown in orange. Murine ADSCs were positive for stem cell markers like CD73, CD90.1, CD105, CD146 and MSC-homing marker CXCR4. A small sub-population of ADSCs also expressed stem cell markers like CD24 and CD133. ADSCs were negative for hematopoietic markers like CD11b, CD25, CD34, CD45 and the endothelial marker CD309. ADSC = adipose derived mesenchymal stem cell; IgG = immunoglobulin G; CXCR4 = Chemokine Receptor type 4.