Homing analysis of ADSCs for EGFP and IFNγ in subcutaneous tumor and lung metastatic models. A) Representative In vivo imaging of green and red fluorescence optical imaging in mouse with subcutaneously co-injected EGFP-ADSCs and RFP-melanoma cells. Focal imaging indicates aggregation of EGFP-expressing ADSCs around the melanoma cells in the tumor. IHC results of subcutaneous tumor sections for EGFP indicate that melanoma cells and ADSCs are not mixed completely in the tumor. B and C) IHC analysis of EGFP + cells. Scale bars =25 μm. Panel B indicates a representative microscopic image of staining for a section of the bone marrow derived from an EGFP transgenic mouse as a positive control. Inlay shows a representative image of staining for a section of melanoma lung tumor from control group. Panel C shows a representative microscopic image of a melanoma lung tumor section after injection of EGFP-ADSCs. D, E, and F) IHC analysis of IFNγ + cells. Panel D) demonstrates a representative microscopic image of a melanoma lung tumor section after injection of IFNγ-ADSCs. Scale bar =25 μm. Panel E) depicts a representative microscopic image of staining for a section of subcutaneous melanoma tumor which indicates juxtaposition of IFNγ-ADSCs with melanoma cells. Scale bar =50 μm. The Inlay shows representative image of TUNEL staining for IFNγ-ADSC/melanoma mixed tumor samples which also confirm position of IFNγ-ADSCs alongside the melanoma cells. Panel F) Representative image which demonstrates both melanoma lung tissue and normal lung tissue. The image indicates that most of IFNγ-ADSCs homed into melanoma lung tissue rather than normal lung tissue. Scale bar =50 μm.