Figure 2

Vinculin suppresses CRC cell invasion and metastasis in vitro and in vivo . (A) Western blot showing vinculin expression in SW620 cells infected with lentiviral vector encoding vinculin and in SW480 cells infected with vinculin siRNA (B). β-actin was used as an internal control. (C) Transwell migration and invasion assays using SW620 cells stably expressing vinculin or control vector. Representative images are shown on the left, and the quantification of 10 randomly selected fields is shown on the right. (D) Transwell migration and invasion assays using SW480 cells stably infected with vinculin siRNA or control siRNA. (E) Representative H&E staining of livers and lungs isolated from mice that received injections of SW620-control-vector or SW620-vinculin-vector cells. Black arrows indicate metastatic intrahepatic or lung tumours. The incidences of liver and lung metastasis in 10 mice are presented on the right. (F) Representative H&E staining of livers and lungs isolated from mice that received injections of SW480-control-siRNA or SW480-vinculin-siRNA cells.