Figure 4

Vinculin positively correlates with membrane-bound β-catenin in CRC. Tissue microarrays of consecutive immunostained sections were performed using specific antibodies against vinculin and β-catenin. (A) Representative images of vinculin and β-catenin protein expression levels in normal and cancer specimens. (B) The expression level of vinculin was significantly lower in CRC tissue species than in normal colorectal tissue species (P < 0.0001, χ² test). The same result was observed for membrane-bound β-catenin (P < 0.0001, χ² test) (C). (D) Membrane-bound β-catenin was positively correlated with vinculin expression in CRC tissues (P < 0.0001, χ² test).