Figure 6

Vinculin regulates CRC metastasis through β-catenin. (A) Western blot analysis of vinculin, membrane-bound β-catenin and nuclear β-catenin in SW620 cells infected with vinculin or control vector (left) and SW480 cells transfected with vinculin or control siRNA (right). (B) Western blot analysis of E-cadherin and vimentin in SW620 cells infected with vinculin or control vector (left) and of SW480 cells transfected with vinculin or control siRNA (right). (C) CRC tissue with high expression of vinculin, membrane-bound β-catenin, positive staining of E-cadherin and negative staining of vimentin. (D) CRC tissue with low vinculin expression, the absence of membrane-bound β-catenin, negative staining of E-cadherin and positive staining of vimentin. (E) E-cadherin expression was positively correlated with vinculin expression as well as (F) with membrane-bound β-catenin in CRC tissues (P < 0.0001, χ² test; P < 0.0001, χ² test). (G) Vimentin expression was negatively correlated with vinculin expression as well as (H) with membrane-bound β-catenin in CRC tissues (P = 0.0274, χ² test; P = 0.0153, χ² test).