THP1 cell characteristics influenced by 10 μM of the dopamine receptor antagonist SCH39166 compared to DMSO control. (A) Proliferation in serum reduced conditions. (B) Colony formation in methyl cellulose. Images show representative wells of DMSO control (left) and 10 μM SCH39166 treatment (right). (C) Cell cycle distribution in serum reduced conditions. (D) Cell migration in 5 μm transwells. (E) DNA new synthesis rate measured via EdU incorporation and SYTOX AADvanced nuclear stain. Ratio of SCH39166 compared to DMSO control treatment is shown. Mean over three (A-C) or two (D-E) independent experiments are shown. Bars indicate standard error of the mean; *p < 0.05. † Non significant differences because of interexperimental fluorescence intensity standard deviation, although the ratio between the two treatments remains similar.