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Figure 1 | Molecular Cancer

Figure 1

From: Integrated analysis of microRNA and mRNA expression and association with HIF binding reveals the complexity of microRNA expression regulation under hypoxia

Figure 1

Hypoxia regulated microRNAs in MCF-7 cells. microRNA sequencing data generated from a hypoxia time course in MCF-7 (16, 32 and 48 h at 1% Oxygen) was analysed and the overlap between the microRNAs found significantly up- or down-regulated at each time point compared to normoxia control (adj.p-val <0.05) has been represented in Venn diagrams (A). The levels of HIF-1α and HIF-2α were measured by immunoblotting across the hypoxia time course (H16h, H32h, H48h) and normoxia (N), with beta-actin used as a control (B). The down-regulation of miR-4521, miR-145-3p and miR-222-5p in hypoxia was validated by qPCR (C). Fold-changes (in linear scale) obtained for each microRNA at each time point relative to normoxia control are represented in boxplots (*significant fold-change compared to normoxia after ANOVA followed by pairwise t-test, adj.p-val ≤ 0.05). The expression of miR-145-5p and miR-222-3p was also assessed by qPCR, confirming a different pattern of expression as compared to their counterpart strands.

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Molecular Cancer

ISSN: 1476-4598