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Figure 5 | Molecular Cancer

Figure 5

From: Histone acetyltransferase-deficient p300 mutants in diffuse large B cell lymphoma have altered transcriptional regulatory activities and are required for optimal cell growth

Figure 5

Total histone H3 lysine acetylation profile in DLBCL cell lines. Total histones were extracted from the indicated cell lines, were trypsinzed, and subjected to mass-spec analysis. For each H3 lysine residue, the amount of the acetylated lysine residue was measured relative to total amount of acetylated plus unmodified residue. Cell lines indicated by red diamonds express undetectable levels of wild-type p300 (RC-K8, SUDHL2) or wild-type CBP (Karpas422) by Western blotting. Cell lines indicated by blue circles express detectable levels of p300 and CBP by Western blotting. A box plot of the fraction of acetylated lysine residue out of total acetylated plus unmodified H3 was plotted for each lysine residue. For cell lines indicated by red diamonds, the mean values and 95% CIs for H3 lysine acetylation were as follows: H3K14 (0.058 ± 0.091), H3K18 (0.012 ± 0.014), and H3K23 (0.231 ± 0.039). For cell lines indicated by blue circles, the means and 95% CIs were H3K14 (0.252 ± 0.141), H3K18 (0.030 ± 0.010), and H3K23 (0.158 ± 0.061).

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Molecular Cancer

ISSN: 1476-4598