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Figure 2 | Molecular Cancer

Figure 2

From: Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models

Figure 2

EMT markers, cell migration and invasion analyses of the HNSCC shSET cells. (A) Western blotting was performed for SET and the EMT markers E-cadherin, ZEB2 and vimentin in HN12, HN13 and Cal27 cells; GAPDH was used as a constitutively expressed protein. The values presented below the bands indicate the shSET/ shControl ratio. (B) Immunofluorescence microscopy was used to detect E-cadherin, vimentin and pan-CTKR proteins in HN12, HN13 and Cal27 cells. (C) The migration assay using the chamber membrane and the invasion through matrigel were assessed in HN12shControl and HN12shSET cells. The results are either representative of three independent experiments or are reported as the means and standard deviations of experiments performed in triplicate (*p < 0.05).

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Molecular Cancer

ISSN: 1476-4598