The effect of NK cell or CD8+T cell depletion on NKG2D-positive lymphocyte localization in tumors. Groups of mice (n = 3 per treatment group) were subjected to one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12. Tumor sections were stained as detailed in the Figure 3 legend. (A) Detection of NKG2D-positive cells in tumors from NK cell–depleted mice, (B) Absence of NKG2D-positive cells in CD8+T cell–depleted mice. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 μm.