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Figure 4 | Molecular Cancer

Figure 4

From: MicroRNA-26b suppresses the NF-κB signaling and enhances the chemosensitivity of hepatocellular carcinoma cells by targeting TAK1 and TAB3

Figure 4

Doxorubicin activates NF-κB signaling and knockdown of NF-κB promotes the doxorubicin-induced apoptosis. (A) Doxorubicin enhanced NF-κB reporter activity. HCC cells transfected with pRL-TK and pNF-κB-Luc or pTAL-Luc were untreated (Ctrl) or treated (DOX) with doxorubicin for 12 hours before luciferase activity analysis. (B) Doxorubicin treatment increased the phosphorylation of IκBα and p65. HCC cells were untreated (-) or treated (+) with doxorubicin for 6 hours before immunoblotting. (C) Doxorubicin stimulated the expression of NF-κB target genes. QGY-7703 cells were untreated (Ctrl) or treated (DOX) with doxorubicin for 24 hours before qPCR analysis. (D) Knockdown of p65 sensitized HCC cells to the doxorubicin-triggered apoptosis. HCC cells transfected with siNC or sip65 duplex were treated with indicated concentrations of doxorubicin for 48 hours before the morphological analysis for apoptosis by DAPI staining. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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Molecular Cancer

ISSN: 1476-4598