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Figure 4 | Molecular Cancer

Figure 4

From: Targeting the prohibitin scaffold-CRAF kinase interaction in RAS-ERK-driven pancreatic ductal adenocarcinoma

Figure 4

RocA mimics PHB-knockdown effects on EMT markers and reverses EMT in AsPC-1 cells. A. AsPC-1 and Capan-2 cells were lysed and then subjected to immunoblot analysis with the indicated antibodies. B. AsPC-1 cells were transfected with PHB-specific (siPHB) or control (siCon) siRNAs. After 48 h of transfection, the cells were lysed and subjected to immunoblot analysis with the indicated antibodies. C. AsPC-1 cells were treated with RocA (100 nM) or DMSO for 16 h, and then subjected to western blot analysis with the indicated antibodies. D. Morphological changes of PHB-knockdown AsPC-1 cells (B) and RocA-treated AsPC-1 cells (C) treated with control siRNAs (siCon) or DMSO. Scale bars, 25 μm. Results are representative of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598