Figure 3

Calcium dependent cell migration and proliferation of RCC cells of different metastatic potential. A, migration of primary RCC cells of 9 patients developing no metastases, lung or bone metastases within 5 years after nephrectomy (3/category) was determined in a Boyden chemotaxis chamber using calcium (10 mM) as chemotactic agent. Migration of RCC cells from patients with bone metastases was clearly increased, p = 0.036. The columns represent mean values and standard error in% of values from non-metastasizing cells (= without metastases). B, primary RCC cells of 9 patients developing no metastases, lung or bone metastases within 5 years after nephrectomy (3/category) were treated with calcium of different concentrations for 30 min. Proliferation of cells from patients with bone metastases, determined by BrdU incorporation, was increased after treatment with calcium, p = 0.099. The columns represent mean values and standard error in% of untreated cells. A, B, using the allosteric CaSR inhibitor NPS 2143 (5 μM) bone metastatic RCC cells were no longer responsive to calcium.