Work flow chart. The central core of the analysis is the comparison between adjacent mucosa and healthy mucosa at transcriptomic (gene expression data) and transcriptional (regulatory network) level. Independent public datasets were used to validate the results. In a second step, tumor tissue was used to search for different DEG patterns. Finally, a crosstalk network was inferred to decipher molecular communication between the tumor and the adjacent gut underlying DEG. Public data was used to elaborate a cellular classification of genes implicated in the crosstalk.