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Figure 1 | Molecular Cancer

Figure 1

From: Hyaluronan-CD44 interaction promotes c-Jun signaling and miRNA21 expression leading to Bcl-2 expression and chemoresistance in breast cancer cells

Figure 1

Detection of HA/CD44-induced JNK and c-Jun phosphoylation in MDA-MB-468 cells. NP-40 solubilized cell lysates were immunoblotted with anti-JNK[pS183] antibody (a) or anti-c-Jun[pS63] antibody (b) or anti-c-Jun antibody (c) as a loading control using MDA-MB-468 cells treated with no HA (lane 1) or with HA (50 μg/ml) for 15 min (lane 2) or pretreated with anti-CD44 antibody for 1 h followed by 15 min HA (50 μg/ml) addition (lane 3) or pretreated with JNK inhibitor (420116) (20 μM) for 1 h followed by 15 min HA (50 μg/ml) addition (lane 4) or treated with non-immune IgG without HA (lane 5) or treated with non-immune rat IgG plus HA (lane 6). [The ratio of phosphorylated c-JNK (a) or phosphorylated c-Jun (b) and c-Jun (the loading control) (c) was determined by densitometry, and the levels were normalized to untreated (without HA treatment) value or non-immune rat IgG (without HA treatment) (designated as 1.00); the values expressed represent an average of triplicate determination of 4 experiments with an SD of less than 5%].

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Molecular Cancer

ISSN: 1476-4598