Figure 4

Evaluation of the status of p53 following treatment of cells with complex 3. A, Upregulation of p53, p-p53 (ser 15) and p21. B, Upregulation of p53, p21 and subsequent nuclear translocation of p53 upon treatment of cells with complex 3 (0 and 10 μM). Scale Bar = 15 mm. Magnification at 40×. C-D, Expression profile of p53 and p-p53 (ser 15) along with the downregulation of p21, Bax and cytosolic cytochrome c upon treatment with 15 and 30 μM of pifthrin-α in presence of complex 3 (10 μM) after 24 h. β-Actin was used as a loading control. E, Percentage growth inhibition of cells in presence of pifthrin-α (0, 5, 15 and 30 μM) and complex 3 (10 μM) with respect to the control (cells without treatment with complex 3). F, Down regulation of p53 and p-p53 (ser 15) in the presence of NAC. β-Actin was used as a loading control. G, ROS generation as determined at 529 nm of cells treated with complex 3 (10 μM) in the presence of PFT-α (0, 5, 15, 30 μM). Values are mean ± S.D and represent one of the 3 representative experiments. *P < 0.05 and **P < 0.01.